The ultrasonic cell disruptor consists of two parts: an ultrasonic generator and a transducer (some are equipped with a soundproof box).
1. Ultrasonic generator: working principle: the signal generator generates a signal of a specific frequency. This specific frequency is the frequency of the transducer. The ultrasonic frequencies generally used in ultrasonic equipment are 20KHz, 25KHz, 28KHz, 33KHz, 40KHz , 60KHz.
2. Transducer assembly: The transducer assembly is mainly composed of a transducer and an horn.
3. Soundproof box: It can effectively reduce the noise during the working process and keep the laboratory quiet. The promotion of ultrasonic cell disrupter in the industry in our country has entered a mature stage, but the application is still not popular enough! The scope of application of this instrument (equipment) is very wide, which is unmatched by other instruments. It is precisely because of this that the market potential of this instrument (equipment) is great, so the number of manufacturers is increasing. This also causes relative chaos in the ultrasonic cleaning industry and the market. You can use eight words to describe "a mix of good and bad, good and bad"!
Classify the ultrasonic cell disruptor. The specific division is as follows:
1. Classification by power The power of the ultrasonic cell disruptor is the customer's index, which determines the quantity, size, quality and effect of the broken objects. Therefore, the manufacturers also attach great importance to this indicator. Under normal circumstances, laboratories, laboratories, research institutes, drug inspection institutes and other scientific research units use low power (generally below 500W); while biological companies, pharmaceutical factories, chemical companies and other production units use power Most of them are around 500W-2000W. Due to the different product structures of various manufacturers, their power marking methods are also different. However, according to the common practice of users, there are generally the following types: 50W, 100W, 150W, 250W, 300W, 350W, 500W, 1000W, 2000W. Generally, the output power of the ultrasonic cell disruptor can be adjusted appropriately according to the needs. The rated operating frequency of the standard ultrasonic cell pulverizer product is 20 kHz. Some ultrasonic cell pulverizers have an automatic tuning function to make the frequency change within a small range.
2. According to the diameter of the probe ("tip"), it is necessary to select ultrasonic breakers with different "tip" heads to process samples with no volume. Due to the different product structures of various manufacturers, the diameter of their "tip" heads are not the same. Generally, "tip" heads range from micro 5mm (suitable for 1ml processing volume) to 25mm (suitable for 1000ml processing volume), with continuous flow probes, and the processing volume can reach 80 liters/hour. Replaceable "tip" heads are used in high-energy applications where wear may occur. When energy is transferred through the "tip" head, corrosion occurs where the traces are left on the metal surface. Over time, there will be slight corrosion spots where corrosion occurs. The "tip" head can be polished with sandpaper or gauze unless it is damaged to a certain degree; when this happens, the "tip" head will have difficulty tuning the frequency, and instead may make a long and sharp noise. Cracks eventually occur. To effectively process a given dose of samples, there are two main factors that need to be considered: "tip" head size and output power. These two factors must be matched at the same time to get the best results. If the “tip” head is large with low power, the “tip” head cannot work; while the “tip” head may be damaged if the power is too large. Please pay attention to the required model accessories when purchasing.
The ultrasonic cell disruptor uses the dispersion effect of ultrasonic waves in the liquid to cause the liquid to produce cavitation, thereby breaking the solid particles or cell tissues in the liquid. The conventional method of use is to put the material to be crushed into the beaker, turn on the power to set the time (vibration time and intermittent time), and put the probe of the crusher into the material. During use, the ultrasonic generator circuit converts 50/60Hz mains electricity into 18-21KHz high-frequency and high-voltage electric energy, so a large amount of heat will be generated during the crushing process, and it is generally crushed in an ice bath.